University of Nagasaki Siebold Campus is located in Nishisonogi-gun, Nagayo-cho. Neighboring Nagasaki City, where the prefectural government is located, Nagayo-cho has been working towards developing itself into a town that fits the slogan of "the city where people want to live, want to continue living, and are glad they live most in Japan" since 2016. Nagayo-cho is famous for its more than 200 years of proud history as a producer of mandarin oranges, and has also recently begun the raising and harvesting of olives for the production of olive oil.

Near to Nishisonogi-gun, Nagayo-cho, where the University of Nagasaki Siebold Campus is located, is Nagasaki City. Nagasaki City holds the largest population in the prefecture and the offices of the prefectural government. Due to the large number of Christian structures and stories of the figures in history who ushered in the end of the Tokugawa era, Nagasaki City is a place with a tangible sense of history. Furthermore, in 2012 it was named as one of the top three night views in the world, along with Hong Kong and Monaco. In addition, Philipp Franz von Siebold, the German doctor who is called "the father of modern medicine in Japan" due to his efforts of education in western medicine and biology, and for whom the University of Nagasaki Siebold Campus is named, lived in the city.